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The template DNA extraction method developed in this study is rapid, sensitive and avoids interference from potential PCR inhibitors and demonstrates the potential of detecting multiple pathogens in milk samples without any enrichment.
In comparison with most methods that exist today, the one described in this study is rapid, cost-effective and does not demand expensive laboratory equipment.
The MAMA method developed in this study is rapid and cost effective compared to DNA sequencing approach and is useful for screening of a large number of K. pneumoniae isolates.
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The results indicate the TaqMan assay method used in this study was rapid, effective and quantitative for monitoring V. vulnificus contamination in seawater and seafoods such as oysters.
The novel nRT PCRs targeting icl2, hspx, and rRNAP1 genes developed in this study are rapid and reliable diagnostic tools to detect viable M. tuberculosis directly from sputum specimens.
Only 17% patients in this study were rapid parasite clearers (PCT100 ≤48 h); these patients had a significantly lower median parasitemia and a higher reported history of previous malaria episodes indicating some degree of prior immunity; none failed treatment during 42d.
PCR using the primers designed in this study is a rapid and sufficient method of screening for bacteriocin-producing strains.
These results suggest that melting curve analysis of PCR approach in this study is a rapid, simple and accurate method in distinguishing the closely related strains of lactobacilli.
The MLVA scheme described in this study is a rapid and reliable molecular typing tool that can be used for further epidemiological studies of bacterial diseases caused by X. arboricola pathovars.
Thus, the novel PMA-mPCR assay developed in this study is a rapid and efficient diagnostic tool for the monitoring of viable B. cereus in food samples and potentially other samples via appropriate DNA extraction.
A particular challenge for this study is the rapid expansion of PBSP in North America.
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CEO of Professional Science Editing for Scientists @ prosciediting.com