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Based on Eggers test and a symmetrical funnel plot, this study is free from publication bias.
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In the present study, the prevalence of LVH (39.7%) among all CKD subjects was lower than in previous studies, because the majority of patients recruited for this study were free of significant cardiac disease.
Mean LVMI and the prevalence of LVH (31.7%) among CKD subjects in this study were lower than in previous studies[35], [36] confirming that the majority of patients recruited for this study were free of significant cardiac disease, consistent with the study design.
Furthermore, intragenic recombination may give rise to false positives in subsequent detection of positively selected codons [65], thus the Recombination Detection Program (RDP3) [66] was used to make sure that all HA sequences used in this study were free of recombination, agreeing with previous observations that intragenic recombination is rare for HA [67].
All subjects in this study were free of confounding neuropathology.
While crossovers confound many studies, this study was free from crossover.
This confirms that the pigs used in this study were free of M. hyopneumoniae.
The patients in this study were free from any inflammatory conditions.
All mice used in this study were free of tumors or any other gross pathological conditions.
All the subjects in this study were free of confounding neuropathology.
The 22 age-matched healthy individuals included in this study were free of acute or chronic disease.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com