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Dried blood spots (DBS) are a useful specimen collection tool in situations where venous access is problematic, however, detection of biomarkers from this specimen type is subject to variation depending on storage conditions and storage time.
Despite the utility of urine specimens for diagnostic purposes, it is unclear whether this specimen type will be equally useful for studies of the male urethral microbiome.
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The number of specimens per patient and the specimen type were decided by physicians.
Clearly the specimen type can affect virus load.
In 13 cases (13/284, 4.6%), the specimen type was not recorded.
However, a wide variety of possible PCR inhibiting compounds or, most often, none at all can be emphasised to be present on such surfaces, leaving any prediction on the PCR inhibition generated by this particular specimen type speculative.
Another limitation of this study concerns specimen type.
However, these specimen types are suitable for DNA typing of ABO Rh genes.
Our original aim was to compare HPV positivity among attenders for screening and defaulters but this comparison is confounded with specimen type.
This approach means that the specificity of either specimen type for any bacterial species will be, by definition, 100%.
The sensitivity of this assay was determined to be less than 85 CFU per reaction for each specimen type analyzed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com