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This requires identification of two flanking CRISPR sites, oriented on opposing strands, and within ~20 bp of the cut site24, and thus the additional targeting density provided by AN19NGG sites would be expected to augment this approach.
This requires identification of the source of the vibrations via an expert system, determination of the required phase angles and amplitudes for the correction forces, and application of the desired control signals to the piezoelectric actuators.
To achieve this requires identification of structures and mechanisms that are either unique to Leishmania or sufficiently different to allow the identification of specific target molecules.
Specifically, whilst each rule requires individuals to strategically respond to others (and thus use social information), in one case this requires identification of informed individuals (expert rule), and in the other, an ability to pool information from multiple individuals (aggregate rule).
Technically, this requires identification of a condition precluding the ability to conceive.
However, this requires identification of effective predictors of response, which are so far lacking.
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Testing this hypothesis requires identification of mRNAs present in the CB.
Full confirmation of this pathway requires identification of a missing mevalonate 3,5-bisphosphate decarboxylase.
Answering this question requires identification of a baseline from which change can be measured and a reasonably long data record.
However, to achieve this effectively requires identification of the additional cost to the National Health Service clinical support services and the development of an appropriately skilled workforce.
Addressing this challenge requires identification of a locus that is moderately conserved across a genus or species of interest but that changes more quickly than the sequence of the 16S ribosomal subunit.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com