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FLC expression is repressed by vernalisation (extended cold) and this repression is maintained in a PRC2-dependent manner following return to warm growing conditions [ 11, 30].
This repression is maintained throughout further progression of EMT [20].
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This repression was maintained at 24 hours post exposure and was still significant in fibroblasts (p<0.05) and, to a lower extent, in keratinocytes (p<0.1) (Figure 7a).
This epigenetic modification is established and repression is maintained by the action of the polycomb group of proteins (PcG) [27].
Glucose repression is maintained in this strain despite a modestly elevated level of Adr1 [6]. Figure 2C shows Adr1 serine 98 phosphorylation in a strain lacking Snf1.
This suggested that nuclear transfer to Xenopus oocytes induces reactivation of genes whose repression is maintained by Xist RNA.
Polycomb-dependent FLC repression is maintained throughout plant development until it is reset during embryogenesis (Gendall et al., 2001; Crevillen et al., 2014).
Csk1 prevents full activation of Pho7 during phosphate replete conditions and repression is maintained even in the minimal UAS construct (280 bp pho1 + pr- yfp + ).
The data can be reconciled by postulating two phases of repression: (1) the acute phase (within 30 min of stress/ND), during which Maf1 associates with Pol III on DNA and represses transcription and (2) the prolonged phase, during which TFIIIB and Pol III are displaced from DNA, the recruitment of new active Pol III complexes is inhibited, and repression is maintained.
In 3 independent time course cultures, treatment with MPA and 8-br-cAMP for 24 hours was sufficient to reduce CLOCK binding to E2 in PER2 promoter by 59%, and this level of repression was maintained over an 8 day time course (Fig. 3 C ).
After nuclear transfer of Xi-GFP TS cell nuclei, Xi-GFP repression was maintained, and no reactivation of Xi-GFP was detected, indicating that resistance to reprogramming also occurs for the imprinted Xi.
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