This protocol comprised the following: (i) a linear DNA mutant library was generated by error-prone PCR or shuffling, and a linear vector backbone was prepared by regular PCR; (ii) the DNA multimers were generated based on these two DNA templates by POE PCR; and (iii) the one restriction enzyme-digested DNA multimers were ligated to circular plasmids, followed by transformation to E. coli.
This protocol comprised T2-weighted (T2-w) sequences in sagittal and transversal planes, a transversal HEMO sequence and a fluid saturation sequence (Fluid Attenuated Inversion Recovery, FLAIR).
28 The authors of this protocol comprise the investigative team of this clinical trial.
This new treatment protocol comprises multidisciplinary cooperation between trauma surgeon, orthopedic surgeon, anesthesiologist and geriatrician, a preoperative workup protocol, a fixed moment of surgery and a postoperative protocol in close collaboration with the physical therapist and nursing home physician.
In the MME group, the protocol comprised two phases: an initial, very rapid opening of the device followed by a second, slower activation.
Treatment duration was 6 or 12 months, depending on genotype, and the protocol comprised a stopping rule at week 12 if early virological response (EVR) was not achieved.
One such regimen, the P6 protocol comprises cyclophosphamide, doxorubicin, vincristine, ifosfamide and etoposide [29].
The suggested protocol comprises of network synchronization and data transmission phases [58].
The protocol comprises the use of attachments and class II elastics.
The suggested BodyQoS protocol comprises of an Admission Control, QoS scheduler and Virtual MAC (VMAC) [61].
