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This protocol complements conventional double TSA-FISH protocols suited to study coexpression of high to moderately expressed receptor mRNA species.
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"THIS protocol cannot be ratified".
Using the optimized experimental protocol complemented with the microarray analysis methodology, as presented in this study, we identify 49 genes as DE between the MNs and dCINs.
The initial values for the two complements cannot be measured using this protocol as condensed sperm is notably impervious to immunostaining due to its highly compacted chromatin state [ 6], resulting in unreliable semi-quantification values for PN0.
These protocols complement, but do not replace, the ICU decision-maker.
Figure 5A (bottom) confirmed the loss of complement activity in rat serum in this protocol, as shown using an in vitro CDC assay in which AQP4-expressing CHO cells were incubated with NMO-IgG and rat serum.
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"THIS protocol can't be ratified".
The law will implement the 2010 Nagoya Protocol, which complements the international Convention on Biological Diversity and aims to guarantee a greater control of the states rich in biodiversity, and their indigenous populations, over how their biodiversity resources are used and an equitable sharing of the benefits.
A combination of the classical CTAB-based protocol [49] complemented with additional purification steps (we added an additional CTAB step with a second incubation and repeated the isoamyl-chloroform step twice more) was applied to extract DNA from all samples.
The original real-time RT-PCR protocol was complemented by the addition of 200 pmol/μL of internal control probe YFP2 (5′-ROX-ATCGTTCGTTGAGCGATTAGCAG-BBQ-3′).
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