During this process, the translocation, scramble and inversion reads were excluded from downstream analysis.
The genes belonging to this group represent functions taking place in all steps of the maturation process (translocation, glycosylation, disulphide bond formation, protein folding, vesicle budding and vesicle transport to the cell membrane) (Additional file 2: Table S2) and do not change during the same transition on glucose.
77 This process involves numerous translocations acquired as a single event from chromosome shattering and re-annealing.
Since folding of the subunit and its assembly into the cell-surface structures are likely co-dependent processes, translocation of the unfolded proteins via the Sec secretion system would allow assembly in the external environment.
If we further consider that the OMM is enriched in ATP, and that non-posphorylatable ERK2 mutants escape from this region with slower kinetics [12], it follows that ERK phosphorylation and dimerization may occur in this compartment, and that this process may potentiate the translocation of the molecule to the nucleus (Fig. 8).
Our group has previously reported a role in this priming process for translocation of the LPS receptor TLR4 to the plasma membrane [ 1].
As the proposed additional level of control of NHE1 through translocation was unexpected, we have compared this process with the well-established translocation of the glucose transporter GLUT4.
However, Tefsen et al. [ 11] showed that, in N. meningitidis, MsbA is implicated in LPS translocation, but is not strictly required for phospholipid translocation because this process was not altered in MsbA mutants.
Thus, additional activation of the Akt pathway is necessary to prevent this process and facilitates the nuclear translocation and activation of β-catenin.
The energy of this process is coupled with outward translocation of protons across the inner mitochondrial membrane, defined as the mitochondrial membrane potential (Δ ψm).
