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The sequences of the primers described in this section are given in Table 2.

RT-PCR was performed using the Qiagen "One-Step" kit and the primers described above.

Transgenic mice (line D) were screened by PCR using the primers described below.

Modified DNA was amplified with the primers described in Table S3.

This construct was then used as template for the in situ mutagenesis reaction using the primers described above.

The HotStarTaq Master Mix system from Qiagen was used for PCR with the primers described above.

AgOXT1 transcript abundance was determined by using the primers described above.

Complete segregation of the mutated chromosomes was confirmed by PCR with the primers described above.

PCR amplification was performed using the primers described in Table  1 (Bioneer, Deajeon, Republic of Korea).