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After this, plates were pre-bent to permit symmetrical compression across the fracture site.
Colonies from this plates were tested for the desired kanamycin-sensitive and sucrose-resistant phenotype by parallel picking.
For this, plates were centrifuged (5 min, 2000 rpm) and 50 μl aliquots of the supernatants were assessed for LDH activity by using the CytoTox 96 kit, according to the manufacturer's instructions.
This plates were then replica-plated on LB-agar added with the appropriate antibiotic, with the aid of a metallic replica plater for 96-multiwell (Sigma, Italy), and grown overnight at 37°C.
After this, plates were centrifuged at 250 × g for 10 min and 100 μl of the cell-free culture was collected and incubated with the reaction mixture from the kit.
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Eel on this plate here".
Cilantro also decorated this plate.
"We need more color on this plate, dude," Chang said.
This plate defines a fixed frame of reference.
Place this plate, with the dough, in the freezer.
This plate, this bite, this breath – right here, right now, they didn't need to make sense.
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