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Expression from this plasmid is driven by UL35 promoter.
Expression from this plasmid is driven by the CMV promoter.
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All plasmids were driven by the immediate early promoter of cytomegalovirus.
The cDNAs in both plasmids were driven by a strong human cytomegalovirus immediate early promoter and each contained an intron to promote high-level expression.
This demand is driven by two factors.
This one is driven by human activity.
Some of this is driven by consumers.
For targeting experiments, CenH3CID, NCenH3CID, NH3, NCenH3Cse4 and NCenH3CenpA were fused at N-terminus of the lacI-DNA-binding domain (see Figure S4 for a description of the constructs), using plasmid lacIST-Topo-TA and cloned into pNHT4 plasmid [36], where expression is driven by the hsp70-promoter.
To verify that the S. pombe Hsp104 homolog exhibits disaggregase activity, the Sp_hsp104+ gene was cloned into a centromeric plasmid in which expression is driven by the native promoter of Sc_HSP104.
In order to confirm that endogenous Hipk1 can antagonize Wnt/β-catenin signaling in another system, we used siRNA strategies to knock-down the human HIPK1 ortholog in HEK293T cells, and then assessed Wnt/β-catenin signaling using a β-catenin-dependent reporter plasmid in which luciferase expression is driven by optimal Lef/Tcf promoters [76], [77].
We next employed a β-actin/EGFP reporter plasmid, where transcription of EGFP is driven by the β-actin promoter, fused to β-actin exon 1 and intron 1 regions (Qin and Gunning, 1997).
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