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This pipeline includes following three parts and is freely available at http://www.ipm.ioz.ac.cn/kang/webpages/locusttranscriptome.html.html
This pipeline includes quality filtering, length truncation (300 bp), dereplication, abundance sorting, OTU clustering, and chimera filtering.
This pipeline includes a preprocessing step that includes vector trimming and masking of repetitive and low complexity sequences.
The output of this pipeline includes, for each orthologous group, a robust single-gene alignment and a most likely tree built in RAxML 7.2.8 (Stamatakis 2006; Stamatakis et al. 2008) with model setting PROTGAMMALG.
This pipeline includes the OCT device and its axial and transverse resolution and sampling, its signal to noise ratio (SNR), the presence of motion artifacts in the data, the performance of the motion correction and merging algorithm, layer and blood vessel segmentation and quasi-rigid registration performance.
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This pipeline, including all results from the mappings of the non-redundant full length cDNA transcripts and Affymetrix probesets is accessible through our Affymetrix probe design tool (http://uqgudmap.imb.uq.edu.au/riboprobe_design/).
This pipeline included the established phenotyping tests described in Table S4.
This pipeline included the adjustment for study-related batch effects to minimize superficial inter-study discordance caused mainly by random noise and technical differences.
The study procedures for this pipeline included: preprocessing, NLP, extraction of coded data and the detection of pneumonia and influenza cases.
For this reason, the first step in the pipeline includes 3′ quality and adapter trimming using the FASTX-Toolkit (http://hannonlab.cshl.edu/fastx_toolkit/) and Cutadapt [ 37] programs, respectivel.
This year, two companies went public last Friday alone, and the pipeline includes published S-1s.
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