Mapping two contigs of the new linkage groups to this region on human chromosome 19 is strong support that indeed these syntenic regions are located on micro-chromosomes in birds.
The 1.5 LOD intervals of the QTLs were considerable shortened with the new linkage maps.
SB created the new linkage map, performed the genome analysis and wrote the manuscript.
New linkage groups were only constructed in case individual markers were linked with a LOD score higher than 4. Construction of the new linkage groups was as described previously [ 16].
Three 81-mer DNA templates were made to test the biocompatibility of the new linkage, each containing a single triazole.
To further evaluate the new linkage groups, we aligned the sequences of the 454 contigs with the SNPs that map to these linkage groups to the human genome.
The order of the markers on the new linkage map agreed well with our published maps (Shi et al., 2009; Long et al., 2011).
ACRW conceived the study, performed phenotypic measurements, conducted QTL analysis with the new linkage map, wrote the manuscript and provided funding for the project.
A total of 932 markers were mapped to the new linkage map generated with the TN DH population using JoinMap_4.0 [ 62].
QTL mapping was conducted as described above using the new linkage map and phenotype data on sex from all 529 individuals in the mapping population.
