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However, many cells and cell lines are either refractory to or adversely affected by transfection, and the transient nature of this methodology renders it unsuitable for the generation of long-term cell lines of the desirable phenotype.
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This methodology rendered two clinical-genomic signatures (see Table 1) containing 7 and 5 prognostic genes for ADC and SCC, respectively (Detailed calculations and intermediate results are shown in Additional file 1).
The methodology renders high-throughput capacity and has been exploited to review large combinations of paths through exhaustive screening, subsequently leading to significant savings in capital and operating costs.
Since up to 99% of microorganisms in environments cannot be cultured by standard cultivation techniques according to taxonomic studies based on 16S rRNA genes [ 14, 15], exploring esterase resources with any culture-dependent methodology renders those resources untapped for uncultured microbes.
In addition, the previous studies differed in their design and methodology, rendering them incomparable to newer Utstein style-based studies.
Despite these promising outcomes, little consensus exists as to whether 1 treatment is more efficacious than others, in part because cross-study differences in methodology render meta-analyses difficult, and in part because many studies fail to document duration of treatment effects or generalization to daily activities.
42 Cooter believes the fundamental incongruity between historical and bioethical methodologies renders historians 'speechless in contempt, silent for political reasons, dumb for fear of offending'.
In our analysis, the relatively low efficiency and yield and high bias generated by the PCR-based methodologies renders them unsuitable for whole genome sequencing and representative amplification of precious DNA.
However, the lower amount of RNA required for this protocol, as compared to the IVT protocol, renders this methodology a highly desirable one for biological systems where sample amounts are limiting.
Comparison of non-persistence between tamoxifen and AIs is difficult as the majority of past studies have often used different methodologies rendering external comparison inappropriate.
But this methodology seems flawed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com