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Failure to find the SAGE tag representing any specific transcript in this library indicates that its expression level is lower than the limit of detection, or that the transcript does not contain an NlaIII restriction site from which a SAGE tag could be generated.
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Characterisation of this library indicated that most inserts were unique and aligned to different regions of the human genome.
The representation of many known and novel miRNAs in this single library indicates the presence of potential miRNAs, which are not yet discovered.
By contrast, a minimum of 28 potential BAC clones containing PPO1 were identified in the library, indicating multiple copies.
Enrichment of podocyte transcript tags SAGE library indicates that other SAGE tags observed at much higher frequencies in this glomerular compared to non-glomerular SAGE libraries are likely to be glomerulus-predominant.
But clues to his personality are scant, although his library indicates considerable erudition, with books by the likes of Dürer, Euclid, Vitruvius, Vasari, Leonardo, Petrarch, and Ovid, and volumes dealing with perspective and optics.
Analysis of structure-activity-relationships within the investigated compound library indicates that NNS and NNN donor chelators show superior toxicity as compared to ONS derivatives regardless of the resistance status of the cells.
The wider gap in coverage depth distribution by Phusion-amplified library indicates uneven and poor coverage of AT-rich regions.
The presence of all three genes in a cDNA library indicates that all three isoforms are expressed.
The lower IC50 of trans-ISRIB relative to the compound in the small molecule library indicates that the library likely contains a mixture of the two stereoisomers.
But the number of obtained clones and the occurrence in a specific library indicates that Myo1F might not be strongly expressed, and primarily expressed in vegetative cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com