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Nevertheless, this latter preparation method also requires a high temperature treatment step to form highly active catalysts in furfural/acetone aldol condensation because the ethoxy residues originated during aerogel preparation need to be removed.
The IC50 values of VAP-Qu, VAP-M and VAP-A for the various breast and bladder carcinoma cell lines were lower than those obtained with VAP-P, indicating that this latter preparation has the weakest effect on viability/proliferation of carcinoma cells among the four VAPs tested.
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For the latter preparation, the freeze-dried residues of the initial starting material (0 h) and at the end of the fermentation (96 h) was treated with a two-step H2SO4 hydrolysis following NREL/TP-510-42618 (Sluitet et al. 2008).
Owing to the latter preparation having a high protein content, the enzyme precipitated on thawing.
These contractile impairments were caused by stable modification(s) since they were observed even after isolation of myofibrils and single-muscle fibres, and the contractile impairments in the latter preparation were not affected by subsequent exposures to an oxidising (t-BuOOH) or a reducing (DTT) agent.
The latter preparation method showed superhydrophobic properties with static contact angles reaching up to 164° and contact angle hysteresis reaching as low as 2.5°.
The latter preparation of enzyme exhibited higher activity and was thus utilized unless otherwise specified.
The latter preparation had a higher protein concentration, resulting in many films with too many overlapping particles.
The latter preparations have the advantage of exhibiting minimal change in Q−A with either time or illumination at 1.7 K.
The latter preparations also revealed unique tubular-like particles.
This latter notion was confirmed by analysis of preparations of human IgG which contained approximately 0.01 0.02% of rHuPH20-reactive antibodies (unpublished observations).
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