Exact(1)
This intermediate allows to couple structurally diverse amino acids at a late stage of the synthesis, representing a significant advantage over most other synthetic strategies that make use of puromycin as key precursor, thereby accepting the drawback that the amino acid at the junction is restricted to O-methylated tyrosine.
Similar(59)
The low stability of the intermediate allows a large free-energy gain upon trimerization, which serves as driving force for rapid assembly.
It is likely that the accelerated kinetics at 24 °C result in more rapid formation of the intermediate, allowing it to build up and influence the observed signal.
This chelation is in contrast to the mechanism for formation of the over-addition product wherein collapse of the tetrahedral intermediate allows a second addition.
The highly reactive Ado intermediate allows the substrate to be readily activated toward reaction through stereoselective H-abstraction.
Density Functional Theory calculations applied to the pre-polymerization adduct and to a possible reaction intermediate allow to choose the best functional monomer that builds up the active sites.
It is likely that excretion serves as a buffer for flux capacity to these intermediates, allowing the cell to adjust formate consumption capacity according to need, rather than maintaining a high constitutive capacity.
A single PCR product was obtained indicating that the PAI excised from the host genome and formed a circular intermediate, allowing DNA amplification across the attP site.
This intermediate step allows us to visualize the result of the two segmentation steps combined.
This study demonstrates a systematic strategy to alleviate the undesirable hydrolysis of thioester intermediates, allowing production enhancement for other biosynthetic pathways with similar issues.
The identification of the different electroreactive species and adsorbed intermediates allowed us to postulate a reaction mechanism for the transformation of AA into dehydroascorbic acid (DHA).
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