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Both genetic and biochemical evidence indicates that Gtr1, particularly when bound to GTP, physically interacts with the TORC1 components, Tco89 and Kog1, and this interaction is diminished under leucine starvation.
On the other hand, although Farp2 also binds to PlexinA1 receptors, this interaction is diminished by Sema3A during axonal repulsion of dorsal root ganglion (DRG) neurons [ 78].
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In MiaPaCa2 pancreatic cancer cells treated with PIAS4 siRNA, the levels of SUMO1 in total cell lysates were decreased as shown by western blot analysis when PIAS4 was knocked down by >90%, Immunoprecipitation experiments showed that PIAS4 and VHL interacted, and as expected this interaction was diminished after PIAS4 siRNA was transfected into MiaPaCa2 cells.
This interaction was diminished when the same two-hybrid assay was carried out in smt3-allR reporter cells or when Zip1C was replaced by Zip1C3N−3R.
As expected, ectopic GFP WWOX can be co-immunoprecipitated by endogenous GSK3 β, and this interaction was diminished when L404 mutated to alanine.
Complexing of FilGAP with FLNa is not simply defined by the binding interface, because the interaction is diminished by deletions of 1) dimerization domain IgFLNa24, 2) FLNa hinge 2, and 3) FilGAP coiled-coil domain.
If the interaction is diminished as in the case of downregulation of uPAR by antisense treatment or interrupted by inhibitors, the signalling complex is turned off.
However exposure to 25 J/m2 of ultraviolet (UV) irradiation resulted in a transient increase in the association of menin and pSer2- and pSer5-RNAPII until approximately 2 hours, after which time the interaction was diminished presumably due to UV-induced RNAPII degradation (Figure 1C).
When we mutated individual serines to alanines, the strength of the interaction was diminished, particularly with S370A mutant, but not completely abolished.
Furthermore, the NURD-H3 interaction is diminished when H3K4 site is methylated [ 19, 20].
Examined by DSC, the non-freezing bound water in the MM membranes was found decreasing with more zeolite loading because the water polymer interaction is diminishing.
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