This insertion, located between SMc03253 and SMc03254, was checked and confirmed by PCR amplification.
The deduced amino acid sequences corresponding to this insertion, located between amino acids 938 and 939 (Burmese strain, M73218), were not very similar, except for 2 conserved amino acids at the C-terminal end.
The presence of this insertion varies between Arabidopsis accessions [ 41], and may contribute to natural variation in SQN expression as detected for other genes tightly linked to transposon sequences [ 42].
Similarity between Prod1 and CD59 is mainly due to the conformation and length of the insertion between β4 and β5, as they both have α-helices in this region and share the position of C7 in the structure.
However, the solution of the insertion between the sacristy and the church caused seepages of water in the point of contact between the two spaces.
The validation of Hel1-331 was done by detecting +/− polymorphism for the insertion between inbreds B73 and Mo17.
Carlsson et al. [ 12] reported that a gap ≥0.35 mm at the insertion between the bone and implant will prevent direct cortical bone apposition on the implant.
In our TEM8 crystal structure, Phe158 is inserted into the hydrophobic core of TEM8, and this insertion enlarges the distance between the α4 and α5 helices (Figure 3B).
This insertion increases the distance between the predicted -35 and -10 boxes of the ptx-ptl operon [ 33].
The TP53 Pinsertiontion (rs17878362) was chosen based on a study by Tan et al. 2006 [ 19], which reported no association between this insertion and the development of radiation toxicity; however, we found that it was important to confirm the lack of association in our study population.
