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Despite this increased background signal, we observed a significant growth enhancement and decrease in WGA binding when the yeast were co-transformed with Gal4AD-CAT and LOC-Gal80 (Figure 5A) or LOC-Gal11 (Figure 5B), and a more modest effect for yeast co-transformed with LOC-Rpt4 (Figure 5C) or LOC-Hap5 (Figure 5D).
Nevertheless, KCl-induced erCaNAR2 oscillations were still clearly observable above this increased background, as were the RCaMP Ca2+ spikes.
Accordingly, the reduced interaction between GCK and GKRP at 25 mmol/l glucose was not significantly detectable above this increased background (Fig. 5b).
This increased background was observed using both the B27 (BD Biosciences, San Jose, CA) and the 4S.B3 (Biolegend, San Diego, CA) clones.
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This increasing background trajectory could be due to incomplete repression of L1 by L2 under the theophylline condition, which we observed in the incomplete repression of Att-1-SFGFP by L2 at steady state (Supporting Information Figure S11B).
However, the increased background due to widening of the slit greatly reduces contrast in the image.
However, the increased background levels present in living embryos have so far precluded such analyses.
For this we increased background variability using results of environmentally stable QTL for all KW determination traits from the IBMSyn4 (B73xMo17) population from Alvarez Prado et al. (2013b).
In this subgroup there were increased background levels of Ki67 and CTLA-4 in unstimulated cell cultures in the majority of individuals.
The overall signal strength was weaker in this cell, which leads to increased background noise and weak signals in the dorsal dendritic branch.
Although no clear band of the corresponding size was obtained after anti-RseP immunoblotting (lower right panel), this may be due to increased background disturbance because of the high expression of RseP-HM.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com