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This helped us sequence these libraries together to a reasonable depth using the sequencing-by-synthesis (SBS) sequencing technology (Illumina inc)., yet the sequences can be traced back to their original stages.
Whilst this helped us to structure our initial thinking, those three categories were not ultimately useful, as they did not fully capture what we felt were the key aspects of the methodologies that were included.
However, another factor is that the N-WASP diVCA construct we use was deliberately chosen because it is only a weak activator (this helped us to engineer mutants that increased activation).
This helped us define our own sound.
"This helped us to be fully prepared," Colonel Pramote said.
"This helped us cover our expansion costs in 2008," Ms. Poe said.
This helped us to focus on school, sports and worthwhile hobbies.
"Along with international sales and production partnerships, this helped us to break the flat funding cycle".
This helped us to learn the potential pitfalls of our design.
This helped us recover work-related emails that were not among the 30,000 produced to State.
And all this helped us appreciate, as deeply as ever, the exquisite variety and fragile sweetness of human life.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com