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We further validated that averaging gene expression data across two different strains in this way strengthens the identification of drug MOA (see Additional file 1: section D).

The result of 80 lines' sequences alignment further validated that re-Bol037156 was a most likely candidate for FOC1.

Our immunohistochemical study further validated that A1BG protein over-expression was seen in most PDAC tissues, but not detected in normal pancreas tissues.

This further validated use of the 21-gene assay with RS being strongly associated with breast cancer death.

Regarding the assessment of methodological quality of each trial, further validated tools that could assess the actual performance or biases of each trial should be developed.

This was further validated using acetylene reduction that measures the activity of bacterial nitrogenase [ 28].

This further reinforces that currently there are no accepted validated risk scores that can be easily applied to clinical practice.

We identified and further validated eleven drugs that activated and stabilized p53, of which four are for the first time described here (Figure 1B and Table S1).

This was further validated in tissues derived from age-matched Ccnd1+/+ and Ccnd1 KI/KI animals (Supplementary Fig S3A), which revealed that phosphorylation of RB at S780 is comparable between +/+ and KI/KI tissues, further suggesting that cells expressing cyclin D1b maintain active CDK4/6 complexes.

This observation was further validated using 3did, which showed that in the interaction pattern of GAPDH and EGFR, the protein tyrosine kinase domain of the latter interacts with GAPDH motifs via SH2/3_1 domains as illustrated in Figure S2.

This validated that Berberine chloride mediated up regulation of p38 MAPK pathway, which was critical for its anti-leishmanial activity.

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