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At the least, this first passage was out of rhetorical step with the rousing ending, which was by far the best formal section of the writing.
This first passage, the book's opening paragraph, establishes one of the great strengths of "Random Family": how close and persistent observation yields telling details.
Equation 1 simplifies as begin{aligned} IG_i = w_i (Gini_D - Gini_i) end{aligned} (2)In this first passage, we can also obtain the frequency of the classes in the entire dataset, which is used in the CAP-growth's extraction phase.
The end of this first passage was defined as P1.
Importantly, this first passage still contained SP-Cpos/CCSPpos progenitors.
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In contrast, PrPres could not be detected in the spleens of 16 mice inoculated with the H-type brain homogenate at this third passage.
Strikingly different phenotypic features were observed in a few mice, which also exhibited shorter survival periods, after this second passage of H-BSE in C57Bl/6 mice.
On this second passage in C57Bl/6 mice using 1% brain homogenates, similar molecular features to those described at first passage (H-type) were observed in mice (26/26) surviving after 469 d.p.i.
The explanation of shortened survivals of mice at this second passage is unclear, but it does not seem to reflect an adaptation of the H-type BSE agent in C57Bl/6 mice since the survival of mice that showed H-type features at third passage was longer.
At this second passage, compared with the 3 other isolates, CEPAD335 (from patient 4) produced smaller plaques on Vero cells.
The survival period after this second passage was again strikingly reduced in comparison to uninoculated mice, after the inoculation of three different concentrations of brain homogenates (5, 1 or 0.2%, i.e. corresponding to 10, 2 or 0.4 mg of brain equivalents per mouse, respectively).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com