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This final preparation contained <3% protein and <1% phosphorus and was used for consecutive chemical and biological analysis.
The supernatant obtained was recovered and the protein concentration of this final preparation (total fraction, TF) was determined by BCA protein assay, using bovine serum albumin as standard.
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This makes the final preparation of the dish on a weekend morning a snap.
We are also grateful to Dr. Rosenfeld for his insightful critique, which proved extremely valuable during the final preparation of this manuscript.
We give thanks for the life of collaborator, coauthor and friend J. William Costerton, who passed away during the final preparation of this manuscript.
We gratefully acknowledge the referees' constructive peer review and thank Ms Dianne Lowe for her assistance in the final preparation of this manuscript.
During the final preparation of this manuscript two reports were published addressing the mutation rate of the SARS-CoV in human populations.
This affects both the ease of formation of emulsions and the stability of the final preparation.
After dilution, the final preparation of recombinant proteins was 0.04 mg/ml.
The purity of the final preparation was confirmed by SDS-polyacrylamide gel electrophoresis.
Following endotoxin removal, the final preparation was filtered through a sterile filter.
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