"Blunt end" addition of dA to full-length products by pol η has been previously observed (34).
These included the expected error-free product as well as those derived from the blunt end addition of dATP, dCTP, and dGTP; the incomplete extension of the primer was also observed.
These included the expected error-free product, as well as those derived from blunt end additions of dATP, dCTP, and dGTP, and incomplete extension.
Briefly, the genomic DNA was fragmented by sonication to 100 300 bp, followed by blunting, 3′-end addition of dA, and adapter ligation according to the manufacturer's instruction (Illumina).
These two products corresponded to correct full-length extension of the primer-template and "blunt-end" addition of dA following correct full-length extension.
Incorporation of dA opposite M1dG accounted for 52% of the total products (including blunt-end addition of dA), and 31% of the bypass products were −1 frameshifts generated by "skipping" the lesion site.
Microtubules are anchored at the centrosome with their minus ends (Bornens 2002) and microtubule growth is regulated by distal plus-end addition of tubulin subunits (McIntosh and Euteneuer 1984).
Minor products were also observed and included incorporation of dA opposite M1dG with "blunt-end" addition of dA (m/ z 836.5, Figure 5F and Table S2) or dG (m/ z 1263.2, CID data not shown).
DNA was fragmented by sonication with the Diagenome sonicator to a mean size of approximately 250 bp, followed by blunting, 3'-end addition of dA, and adaptor ligation according to the manufacturer's instruction (Illumina).
In this study, we totally observed 499 and 409 kinds of 3' end addition fragments in G1245N and G1245T library, respectively.
