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This deletion greatly reduced the bias, showing that these deleted sequences also play a major role.
The targeting plasmid used to delete hprt exon1 was called pΔHprt to indicate this deletion.
As it happened, Congress removed the conscientious objector clause, but this deletion should not change our understanding of the remaining language.
Towards this, deletion constructs of pCREBBP, viz.
This deletion comprised exons 1 5.
In addition, in silico analysis using MutationTaster supported the pathogenicity of this deletion mutation.
Therefore, this deletion is strain-specific in A. oryzae and might not affect ustiloxin B production.
DNA sequence analysis revealed that this deletion may be the result of microhomology-mediated end joining.
This deletion was thought to be responsible for the slender grain in Basmati 385 compared to HJX 74.
This deletion was further confirmed by PCR amplification of DNA, mRNA expression, and DNA sequencing (Fig. 1B D).
This deletion did not appear in the inducible clones, with promoter sequences identical to that of the wild-type strain.
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