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For the remainder of this paper, this conjugation will be referred to as GNR/4MBA@Anti-ICAM-1. 4MBA, BSA, glutaraldehyde, and PAH used in this study were purchased from Sigma-Aldrich.
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Therefore, the covalent conjugation will lead to a decreased binding efficacy of the antibodies.
Our recent results show that the non-covalent conjugation will provide us a new way in semi-synthesis of artificial metalloenzymes.
These results suggested that integrating icariin into scaffold by chemical conjugation will increase the quality of formed cartilage, and may decrease the risks of excessive release, therefore is valuable in cartilage tissue engineering.
The use of comparatively stable amide linkage for TPP conjugation will allow Mito-DCA to navigate into the mitochondria.
Taken together, the recombinant nature of Protein Z production combined with the ease of cross-linking will greatly expand the accessibility and utility of this conjugation method.
Thus, this conjugation modality often gives multiple ADC species with variable DARs and conjugation sites.
An N297Q mutation prior to this conjugation provides two more reaction sites (DAR = 4).
This conjugation compensates for the minus sign in (3.5) and in higher-order Clifford products.
This conjugation with AsA (which has strong redox properties) increases glycoside stability.
This conjugation is usually considered as a detoxification procedure.
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