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Following this, cells were washed with PBS (pH 7.4, 0.1 M) and fixed with 4%% paraformaldehyde.
For this, cells in 96-well plates were grown to 80% confluency.
For this, cells were incubated with QDs for the predetermined time intervals and then washed to remove any unbound QDs.
Following this, cells were washed twice with cold phosphate buffer saline (PBS) and were detached using cell scrapers.
In this, cells with self-renewal potency or 'stem-ness' provide genetically diverse units of evolutionary selection in cancer progression.
For this, cells were incubated with QDs for 1, 2, 3, 4 and 5 h and then washed to remove any unbound QDs.
To achieve this, cells from different sources, growth inducing molecules, and a biomaterial-support called a scaffold are used in isolation or in combination to reconstruct organs.
Following this, cells were seeded onto surface of samples and a negative control (i.e., MG-67cells only in the cell culture medium) then placed in a 24-well tissue culture polystyrene (TPS).
The phrase they use is this: Cells that fire together, wire together.
Using a mechanism like this, cells are able to actively regulate flux through the Golgi.
After this, cells were washed in Ca/Mg Phosphate buffered saline and used for confocal microscopy.
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CEO of Professional Science Editing for Scientists @ prosciediting.com