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The central part of the lambda genome is not essential for the virus to replicate in Escherichia coli, so this can be excised using an appropriate restriction enzyme, and inserts from donor DNA can be spliced into the gap.
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Indeed, the circularized form of the element was detected by PCR (data not shown) suggesting that this element can be excised from the chromosome and may be transferred by conjugation with subsequent integration into the recipient.
However, this oncogenic DNA can be excised from the transferred DNA and replaced by any gene of interest for A. tumefaciens-mediated engineering of a widearray of plant species.
If American negotiators are unable to address trade rules, the round will almost certainly fail.The Bush team now hopes this "killer" amendment can be excised from the final fast-track legislation that will emerge after negotiations to reconcile the Senate bill (once it is passed) with the House version.
The oxidized base 7,8-dihydroxy-8-oxoguanine (8-oxo-G) is one of the mutagenic products of oxidative DNA damage; however, misincorporated 8-oxo-G can be excised by the base excision repair (BER) pathway [ 8].
Although ICLs can be excised by other pathways, nucleotide excision repair is a primary pathway for their removal from DNA.
Where an apparently meaningful development isn't". This is the extra plot that can be excised painlessly, without harming the host organism.
Again, this suggests that the entire cluster can be excised or inserted as a functional unit.
After this fragment is inserted, the marker can be excised as a result of recombination between the direct repeats.
Cerebral abscesses can be excised and this seems to lower the mortality of the patient compared to solitary drug treatment with or without aspiration/drainage, depending on the size and growing behaviour over time [ 28].
PCR and sequencing demonstrated that different fragments of the PAI can remain in the chromosome after excision, or chromosomal fragments can be excised together with the PAI.
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CEO of Professional Science Editing for Scientists @ prosciediting.com