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This assays appeared to be more sensitive than the MTT assay, since even at this low dose, JS-K resulted in significant cytotoxicity and/or loss of viability in the TR1 knockdown cells (∼45% viable) compared to the cells with endogenous levels of TR1 (∼68% viable, Figure 7A and 7B).
This assays presented here including the Cajal body localization and LacO array recruitment are very indirect and are in vivo.
As expected, we observed a certain degree of donor variability in this assays with e.g. IFN-γ induction ranging from 500 pg/ml to 5ng/mll.
A C. difficile toxin assay for both CdtA and CdtB was approved in 2007 in Japan [ 20, 85], however, prior to this assays for only CdtA had been used, and many studies claiming to have excluded CDI by toxin assay might have failed to detect CDI producing only CdtB.
This study was carried out as part of the training programs in TB laboratory diagnosis for Mozambique and other Portuguese speaking countries, created and implemented to assess the usefulness of this assays for routine identification of M. tuberculosis in the network of hospital mycobacteriology laboratories in these countries as well as in Portugal.
However, we and others have demonstrated that signals from endodermal cell lines can influence myocardial differentiation from both mouse and human embryoid bodies (EBs), and because of this, assays that utilize embryonic stem (ES) cells and endodermal cell lines provide excellent in vitro models to study early cardiac differentiation.
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"Like, 'Did you do this assay?
This assay was carried out in triplicate.
This assay was conducted without antibiotics.
With this assay, multiple samples can be handled in parallel.
This assay was performed as described in ref.31.
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