Array details can be found at the NCBI GEO (GPL8707 and GPL8954).
We assessed the DNA methylation profile of 1,505 CpG sites (associated with 808 genes) in 15 ovarian cell lines and 27 primary tumors (15 serous, 9 endometrioid, and 3 clear cell), using the Illumina GoldenGate Cancer Panel I [16] (see Supplemental File S1 for methylation array details).
GeneChip® Soybean Genome Array details are available at the Affymetrix® website [ 26].
An experiment record shows the provenance, experimental design, protocols, sample information, array details, quality assessment and a list of array measurements.
Therefore, these three arrays found a pathogenic CNV in 14.3% of patients (3/21, one patient had two CNVs), which corresponds to the results obtained on the CGX-12 array (details in the next section).
The purified aRNA was quantified using a NanoDrop spectrophotometer and 4 μg were fragmented to a uniform size, then hybridized to a custom Bovine 90 K array (see [ 27] for array details).
Diks et al have described the design of this array in great detail [16].
Spotting of PCR products from 384-well plates was done using either a GMS 427 (Affymetrix) or a MicroGrid II (BioRobotics) robot (see arrays details below), onto precut Nylon membranes (Pall) of size 72 × 18 mm.
The design and performance of this array are described in detail by Hodgkinson et al. [ 14].
Details of this array have previously been published (Whitaker et al, 2010).
This feature is a PAM with six states; zero, one, two or three CCHC arrays (detailed using sequence logos).
