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This allows targeting to take place at a street or postcode level.
Although past mammary studies focused heavily on the use of hormonally responsive promoters, such as MMTV and WAP, to direct gene expression or ablation, the use of K5 and K14 promoters has become increasingly popular as this allows targeting of genetic changes to developmental mammary stem/progenitor cells (Berton et al. 2003; Choi et al. 2009; Gu et al. 2009; Taddei et al. 2008).
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This allows targeted recruitment of key cofactors such as CRY, PER, HDAC3 (repressors), along with SRC1-3 and PGC1 (activators), which coordinate a cycling pacemaker gene network.
Based upon a linear relationship between amplification rate and amplicon quantity, this allows target quantification to be conducted using linear regression analysis.
SureSelect is a capture protocol based on the in-solution method developed by Gnirke et al. This method allows targeting of regions of the genome in custom designed reactions by using cRNA baits.
Given that this strategy potentially allows targeting of promising cancer therapies to hypoxic tumors, it is surprising that it has not been more widely employed, although there are a few reports in the literature.
But this is different because it allows targeting by individual user.
This system allows targeted inclusion of a single coumarin-based fluorophore with high quantum yield and high environmental sensitivity to pH and solvent polarity.
This NGS approach allows targeted sequencing of all disease genes listed in the Online Mendelian Inheritance of Man (OMIM) database.
This optimized approach allows targeted transgene integration into safe harbor sites for more predictable and robust expression and enables the straightforward generation of disease-corrected, patient-derived iPSC lines for research purposes and, ultimately, for future clinical applications.
To study the ability of anti-CD134 liposomes to reach the potentially auto-reactive CD134+ T cells in vivo, the active disease model was employed, because this would allow targeting of the target T cells during priming in situ; that is, in the secondary lymphoid organs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com