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Thereafter, we combined this alignment with the aligned MAEL domains based on fold recognition results and predicted secondary structures.
To retrieve even more distant UlaGL sequences, we first constructed a structure-based sequence alignment using the superimposed homologous structures identified with PDBeFold [ 30] (see below) and used this alignment, with 5 sequences and 232 aligned amino acid residues, as seed for the calculation with HMMER v. 3.0 (http://hmmer.org/) of a profile hidden Markov model (HMM) [ 33].
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However this alignment with the Liberal government's approach to state policies reflected highly instrumental and strategic decisions rather than a wholesale endorsement, since we know from other interview analyses that advocates privately prioritize and are motivated in their advocacy work by the conviction that childcare is a women's equity and justice issue (Langford and Richardson 2013).
The optimal evolutionary model for the family was calculated from this alignment with ProtTest [ 64].
A majority-rule consensus sequence was taken from this alignment, with the minimal requirement of 2x coverage.
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It seems that the points with velocities between 10 and 15 m/s also have this alignment, although with larger scatter.
It was also found that surfactants cause the drops to align more in the flow direction and that, for both clean and surfactant-covered drops, this alignment increases with viscosity ratio.
This alignment fits with Stiggins' contrast between 'assessment of learning' and 'assessment for learning' [ 30].
For this, alignment positions with more than 50% gaps were eliminated using trimAL before conducting the analysis.
The MOLPHY package [ 71] was used for phylogenetic reconstruction based on this alignment (positions with gap fractions larger than 0.1 were removed).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com