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This alignment includes 71 B. schlosseri sequences and the three best outgroup sequences belonging to Botrylloides leachii and Botrylloides nigrum.
This alignment includes the genomes of human (Homo sapiens, version NCBI 36.1, March 2006), chimpanzee (Pan troglodytes, version PanTro 2, March 2006), and rhesus monkey (Macaca mulatta, Baylor College of Medicine Human Genome Sequencing Center, version v.1.0 Mmul_051212, January 2006).
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This alignment included 25 dinoflagellate taxa, with 1,229 unambiguously aligned bases (Additional file 2).
We excluded the most divergent sequences from this alignment, including those from sponges that lack the Irx specific domains.
One included 47 representatives from the broadest diversity of sequences available; this alignment including a partial sequence from Cyanophora (regions A-G).
A Bayesian phylogenetic tree produced from this alignment included four sub-clades each containing one of the four functionally characterized latrotoxins from L. tredecimguttatus, together with conserved orthologs assembled from our L. hesperus short-read data.
Cand9 also had a structural alignment to the RFAM family RF00708, but this alignment included only 12 bases of the original read, and in spite of the fact that the family has been characterized in human, the alignment did not cover the whole consensus sequence.
Phylogenetic analysis was carried out using this alignment including all Su var)3-9 partsin parts except the common region with eIF2γ and the region 2 (Fig. 2) [see Additional file 2] and implementing BI, ML, MP and weighbor methods (see Materials and Methods).
In total, 13 positions in the alignment included gaps (Additional file 2: Figure S2).
When the vertical alignment includes a tangent gradient, a profile sketch might be useful.
Based on the American Association of State Highways and Transportation Officials AASHTOO) soil classification systems soil along the proposed alignment includes A 2 4, A 2 6, A 2 7, A 7 5, A 7 6, and A 6 (at one location).
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