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The availability of this additional assay will allow us to examine the extent to which MIRU further differentiates genotype clusters on the basis of RFLP and spoligotyping.
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Further, those additional assays often require a large amount of sample, are labor intensive and not always successful [ 11].
Therefore, this additional BSS assay can be used to both positively identify and study the methylation status of chromosomes with the 10A176T haplotype.
However, target cells are usually labeled immediately prior to assay, which inadvertently introduces additional assay variations due to differences in target cell conditions and labeling/handling processes.
A customized software program provided two additional assay metrics, Quantification Titer (QT) and Threshold Titer (TT), both of which demonstrate acceptable accuracy, precision and dilutional linearity.
As an additional assay of miRNA activity, a second set of transgenes was constructed and tested.
We measured cell viability with an additional assay, using the Cyquant cell proliferation dye.
Additional assay control included analysis of the same known positive and negative serum samples with each run.
Yersinia sp. biofilms that bind the C. elegans exterior provide an additional assay for the nematode surface.
An additional assay using 5S LT1-specific primers, in which PCR amplification indicated DNA methylation status (Figure 3A), also supports the hypothesis that the hypermethylation is siRNA-dependent.
Additional assay results, assay protocols, and synthetic procedures and characterization data for 4, 66, and 68.
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