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To appreciate the contrast between K-S test results of the first and third level scores, we plotted the quartiles of the normalized L1 scores (L1) and L3 scores (L3) of AON target sites for AONs in each grade of Sets A and B for comparison (Figure 4A and 4B respectively).
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Three fourth level scores were developed for this analysis.
Again, the fourth level scores resolved this conundrum in a similar manner as the first example.
Statistical tests as in Table 1 were applied to each of the three fourth level scores.
To demonstrate the correlative power of the fourth level scores, three common examples in which only the fourth level scores can differentiate AONs in Set A (Figure 6) are discussed.
The three fourth level scores (L4_AVG, L4_AND and L4_OR) were next applied on every group of consecutive nucleotides.
For every AON target site analyzed, the three fourth level scores were calculated for every group of three consecutive nucleotides at 3' and 5' ends of the target site.
Two-sample Kolmogorov-Smirnov (K-S) test was used to test for statistical differences and significances of the first, third and fourth level scores in their abilities to differentiate AON efficacy and efficiency between any two AON samples.
The three fourth level scores were calculated only for those groups of consecutive nucleotides meeting the following criterion for analysis: every nucleotide in the group having an engaged score of at least 0.1.
For instance, in the case of AONs, each of the fourth level scores of the groups of nucleotides at the target site 3' end was compared with the 5' end using K-S tests.
Results of the parametric study show that lean loading is an influential factor in determining first level EHS scores, process costs and environmental impacts and a trade-off is observed between EHS and LCA scores for some of the solvents.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com