Exact(5)
Dead cells are already detected after the first dosage of drug, reaching a peak after the third inoculation with CDDO-Im (Figure 4A).
This is a promising result, because in a previous study using recombinant vaccinia expressing AHSV-4 VP2, significant neutralising titres were not observed in vaccinated horses until after the third inoculation [28].
A third inoculation was again performed with the second lysate (8 ).
The third inoculation method, snipping off the distal approximately 1/3 of the young leaf with contaminated scissors, proved to be the least robust method, and PR gene induction was delayed by 24 hours (Fig. 3).
Two weeks after the third inoculation, ten mice in all groups were challenged intraperitoneally with 1 × 10 tachyzoites of the virulent T. gondii RH strain and the survival time were recorded daily until all mice were dead.
Similar(55)
The second and third inoculations were performed on days 24 and 36 with Freund's incomplete adjuvant (Sigma), and the fourth inoculation was performed on day 41 without any adjuvant.
In the first inoculation, 10 A549 cultures were inoculated with samples from 30 patients (three patients per culture dish).
Seven to ten days after the first inoculation, all the plants were inoculated for a second time as above.
One hundred and eight hours after the first inoculation, the ear bases, containing all inoculated spikelets of DON-treated and mock ears, were sampled separately and immediately shock-frozen in liquid nitrogen.
In the second inoculation, samples from these 15 patients were individually inoculated into A459 cells.
For CVYV, plants were inoculated a second time five days after the first inoculation.
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