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Third generation sequencing.
Second and third generation sequencing techniques now allow the sequencing of thousands or millions of barcodes, or even entire genomes, in parallel and such high-throughput technologies are available to virtually every laboratory.
The panel of IHC antibodies will strongly depend on the results of third generation sequencing.
These include paired-end and mate-pair technologies [ 5], third generation sequencing [ 6], and optical mapping [ 7].
In addition, with the availability of third generation sequencing technology, this kind of data will soon become widely available.
Therefore, we then included the PacBio RS sequencing platform, which is a third generation sequencing technology and can produce reads longer than large repeats within the genome.
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Single Molecule, Real-Time sequencing technology (SMRT) is the third-generation sequencing technology developed by Pacific Biosciences (PacBio).
This general approach will be adaptable to improving second- and third- generation sequencing techniques that may allow even longer amplicons to be completely sequenced [ 25, 26].
However, for tSMS and more generally third-generation sequencing, the most common type of sequencing errors are indels, particularly deletions [ 26].
Furthermore, third-generation sequencing technologies which can generate deep sequencing data within hours are now available [48], [49].
This general approach will also be adaptable to improving second- and third-generation sequencing technologies that may allow longer amplicons to be completely sequenced [ 25, 26].
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