To increase allele specificity, we gave an artificial mismatched nucleotide near the 3' end (second or third nucleotide from the 3' end) of the allele-specific primer (lowercase with underlined nucleotide in Figure).
His winner, 90 seconds from the end of normal time, provided an appropriate climax.
They can be miles ahead 10 seconds from the end and win nothing.
"Thirty seconds from the end, and it all goes up in air".
The Rangers even scored a goal of honor, 32 seconds from the end, when Wojtek Wolski ended the shutout.
And when it was over Saturday afternoon, only 3.6 seconds from the end of overtime, the Rangers stood alone, victorious.
The initial display of spontaneous celebration came a minute and seven seconds from the end of a national championship game that had felt decided for days.
The first ball went over it inside half a minute and, 30 seconds from the end, Miller nodded the last, unchallenged, in an innocent piece of shrugging mischief.
Walsh took his goal tally to six from seven attempts before wrapping up the scoring with a drop goal 90 seconds from the end.
The game did not deserve a winner but it got one, the substitute Costinha rising magnificently seconds from the end to head home Figo's free-kick.
Seconds from the end Adam took a corner on the right and Robert Huth transferred the ball to Steven Nzonzi, whose header hit Routledge at point blank range.
