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Porcine MSCs were cultured to a thin layer of cell sheets via osteogenic induction.
Each wash consisted of removing spent media, adding fresh imaging media, and incubating the cells for 5 min. After each wash a thin layer of cell imaging media was added and wells were rapidly imaged.
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A second thin layer of cells, constituting the endoderm, lines the gastrovascular cavity.
As the synovial membrane (the thin layer of cells lining the joint) becomes inflamed, enzymes are released.
The researchers wanted to look at the effect smoking has on the thin layer of cells that line blood vessels and are known as endothelial cells.
Further differentiation produces a thin layer of cells, called the hypoblast, between the inner cell mass and the cavity.
The inner surface of every blood vessel is lined by a thin layer of cells known as the endothelium.
The ideal healing would be limited growth -- a thin layer of cells to carpet the stent-injured area on the inside wall of the artery, rather than thicker scarring.
Just like blood capillaries, the walls of the lymphatic capillaries are composed of a thin layer of cells, the endothelial cells.
The findings, published in Nature Communications, could also apply to prostate, liver, skin and many other cancers that arise from the epithelium, the thin layer of cells that lines the outer edge of many bodily organs.
Under normal physiological conditions the synovial lining consists of a thin layer of cells with phenotypic features of macrophages and fibroblasts.
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