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Donor corneal stromas were cut to 120 200 μm thickness slices using a microtome and then decellularized.
In contrast, a cone shaped STEM beam produces images of thickness slices from the sample.
Further differences between the detection modes are caused by the convergence of the electron beam to form a local probe, which forces a serial image acquisition of thickness slices with an increased spread of the electron beam in z-direction because of electron channeling in zone axis orientations of the samples.
The experiments were performed using intact tissue (Hb and IPN) or hippocampal (300 μm thickness) and parietal neocortical (400 μm thickness) slices; the slices were cut using a McIlwain tissue chopper (The Mickle Laboratory Engineering Co. Ltd ,Guildford, Surrey, UK).
Cut all ingredients into medium thickness slices & separate them.
Place 3-5 (depending on desired thickness) slices of cooked Black Forrest Ham on the bacon.
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The 543-nm laser was always used with a smaller detection pinhole diameter than the 488-nm laser to obtain the same optical slice thickness (slice thickness typically between 0.5 1.0 µm).
The reconstructions will probably differ in their slice thickness, slice overlap, filter etc.
The reconstructions differ in their slice thickness, slice overlap, filter, etc.
The calculations used for the renal cortical and whole kidney volume are as follows: Whole kidney volume = ∑ Area of the kidney in each slice × slice thickness (slice interval ) Renal cortex volume = ∑ Area of the cortex in each slice × slice thickness (slice interval ) Chronic kidney disease is regarded as public health problem throughout the world [ 4].
Transmission electron microscopy (TEM) on vertical through-thickness slices reveals highly localized thick oxides (∼50 nm) in the area of large cyclic stress, but not in control specimens.
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