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Additionally, ratio-based FISH predictors are advantaged as they are less dependent on the thickness of slides or nuclear truncation (see additional Supplementary Technical Notes).
To eliminate errors caused by variances in thickness of slides and cellularity of areas investigated, the ratio of apoptotic to mitotic indices was calculated in each tumour.
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As commonly understood in slope stability evaluations, the slope angle is much more important than the thickness of sliding mass.
Confocal images were obtained with a Leica SPE upright confocal microscope with 63×N.A. = 1.4 objective and the thickness of optical slides was set to 0.3 microns.
Other discrepancies may also be because of differences in the sample materials or the thickness of the slides.
In this test, with consideration of the stress capacity of the ring shear apparatus, the initial slope angle of the Donghekou complex slide was maintained, while the thickness of the sliding mass was reduced significantly.
The thickness of the sliding material is 50 m in the main tension trough and decreases to several meters in the direction of sliding.
The initial normal stress and shear stress were obtained from the slope angle, thickness of the sliding mass, and the unit weight of the soil/rock.
The average thickness of the slide mass in the source area is about 75 m, with the maximum thickness of 110 m, and the total volume of the Donghekou rockslide is about 69 million m3.
As we all know, if the thickness of a sliding mass on a slope is much smaller than the slope's length, the slope can be called an infinite slope.
Each sample was tightened using a binder clip with a maximum binding force of 40 N for 2 mm thickness of two glass slides.
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