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The thickness of ears was measured at the indicated time points.
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The ear swelling was followed by measuring the ear thickness of both ears at three time points and calculating the mean per ear.
After 5 days the ear thickness of both ears at three points each was measured and mice were challenged with DNFB 0.2% and treated with [44AANA47]-CCL5/vehicle/dexamethasone, as reference compound at 0.5 mg/kg, 30 minutes after challenge via i.p. injection.
The thickness of the ears was measured before and 6 h after induction of inflammation.
To understand deeply the 5EL treatment, we performed determination of the relative thickness of rabbit ear hypertrophic scar.
Relative thickness of rabbit ear hypertrophic scar before and after the treatment was measured by caliper method.
The thickness of the ear is measured 24 h later to determine the level of swelling [12], [13].
Before and at 15, 30, 60, and 120 min after edema induction, the thickness of each ear was measure with digital vernier caliper.
The thickness of the ear was measured before and 24, 48 and 72 h following challenge using a micrometer (Mitutoyo, Kawasaki, Japan).
16 gauge and about 10mm long (3/8") is a good size; the length allows for swelling, which can easily double the thickness of your ear.
In addition, a DTH test was executed and all rats showed a good DTH response with a significant (P = 0.001) increase in ear thickness of the right ear at 6, 24, and 48 hours after injection compared with the control left ear) and compared to control rat ear's injected with saline (data not shown).
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