Due to the mechanism of cell division, dividing cells are, on average, thicker than nondividing cells, thus leaving a window of cell thickness spanning from 6-7.6 μm (cytoplasm region) and 4.1-8.3 4.1-8.3leus) amenable for comparison.
Some of the Grp94-positive and calreticulin-positive myotubes also displayed strong labeling for CHOP [ 30], whose staining localized either in the nucleus (thick arrowhead), in the cytosol (thick arrow), or at both locations (single arrowhead).
One of the mission goals was to image the crater made by the explosion, but the dust cloud was so thick that the nucleus surface could not be seen through it.
(The Figure 7A panel illustrates a region of severe PR degeneration in which the ONL is only one nucleus thick).
The spores showed a thick band like nucleus at one pole and stained positively with modified Gram Trichrome stain (figure 7), 1% acid fast stain (figure 8) and variably with GMS.
To study the level of glutathionylation in the different regions of the lens, two human lenses (32 and 56 years) were dissected into outer cortex (about 0.5-mm-thick layer from the surface), inner cortex (about 1-mm-thick layer), and nucleus regions.
The cells were typically only ∼1 µm thick (except at the nucleus), while the lateral dimensions were ∼20 µm.
Some spores did not take up the stain and appeared blue, but all of them showed a thick band (arrowhead) like nucleus at one pole (Fig. 4a).
27 Briefly, parasagittal brainstem slices (200 μm thick) containing the medial nucleus of the trapezoid body were prepared from 7 to 10 days old male or female WT mice, or their Cln1 −/− littermates using a vibratome.
Control slices were characterized by the compact division of the nucleus ambiguus (cNA), a thick dorsal inferior olive (IOD), and a minimally developed principal loop of the inferior olive (IOPloop).
