Fifty German divisions advanced through thick, freezing fog and overran the partly finished British defences.
This strategy was followed by Takimoto et al. [ 79]: a 5 mm thick freeze-dried collagen sponge was wrapped around a silicone tube and implanted into dogs to recover a 10 cm defect.
If you want the drink thicker, freeze the raspberries.
A transparent, eight-inch-thick, frozen moon.
To confirm IBAT activation, biopsies were taken for histological evaluation (hematoxylin and eosin [H&E] staining) using 20-μm-thick frozen sections.
For 3D aggregates, 5 µ-thick frozen sections were mounted on glass slides.
8- to 12-μm-thick frozen sections were used for immunostaining as previously described (Gruber et al, 2011).
Frozen spinal cords were embedded in cryomatrix compound (Thermo Fisher Scientific, Waltham, MA, USA), and 7 μm-thick frozen sections obtained.
For immunohistochemistry, 6- to 8-μm-thick frozen sections were cut, immediately fixed in cold methanol for 10 min, air-dried, and washed in PBS.
For LMD, 5 μm-thick frozen sections were fixed in 70% ethanol for 30 s, stained with hematoxylin and eosin and de-hydrated as follows: 5 s each in 70%, 95% and 100% ethanol.
Overnight the air was thick with freezing fog, but now the sky is flung wide open, a bright bluebird of a day, as sunlight glitters on the drifts and the rime frost that has grown in shards from the rough grasses.
