For real time qRT-PCR, intact male and female hpg mice and WT littermates aged P10, P30, P45 and P60 (n = 3 6 per group) were killed, brains were removed and coronal sections of brain tissue (1 mm thick) encompassing the regions containing either the ARC and AVPV were obtained with the aid of a 1 mm coronal brain matrix (Braintree Scientific, Braintree, MA).
Human skin Damen and Mier (1982) found no 7-ethoxycoumarin O-dealkylase (ECOD) (prototypical CYP1A and 2B substrate) activity (<3 fmol/min/mg tissue) in human skin (0.2 mm thick, encompassing the whole epidermis, but only part of the dermal adnexae) homogenate (while in rat and mouse skin homogenates, there were clear-cut activities of 12 ± 8 and 48 ± 23 fmol/min/mg tissue, respectively).
Like the two other paintings by Najafi on view, it's a menagerie of both subject and media – thick oils encompass a flock of lenticular images, the multilayered graphics that appear to shift along with one's viewing angle; the primary visual effect Najafi employs is stereoscopic ("two and a half dimensional," in his nice description).
The Ediacaran Bambuí 1st-order sequence (i.e., Bambuí Group) covers most of the intracratonic São Francisco basin (southeast Brazil) and encompasses thick packages of carbonate and fine- to coarse-grained siliciclastic strata.
The veil of suspicion is so thick, so all encompassing and, in Hunter's case, so unforgiving.
In addition, we have shown in a previous work that the presence of thick plastic walls encompassing the target object has an important effect on the evaluation of PET-AS methods [7].
Sweat remained on the lam Saturday, and about 1,200 searchers focused intensely on 22 square miles encompassing thick forests and heavy brush around where Matt was killed.
For each animal, 10 12 cryostat microtome sections (40 µm thick) were collected encompassing the entire rostral preoptic area and were arranged in chronological order.
Because their careful observations were based on serial thick sections to encompass the entire target nuclei, their irrefutable data are much more reliable and convincing than chance observation on routine single section used in other studies.
To calclulate the CLI we used the following equation: CLI = (i/[i + I])/(a/[a + A]) where (i) represents the pixel intensity contained in a 0.625 µm thick cortical band encompassing the cell edge, (I) the cytoplasmic pixel intensity, (a) the cortical region area and (A) the cytoplasmic area.
