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They were also sorted according to parity: they were sorted into different categories if they had previously already given birth to other children or not and, if so, how many.
In order to quantize the expression values of every gene they were sorted in ascending order and the first and last values of the sorted list were discarded as outliers, then the upper 50% are converted to 1 and the lower 50% is converted to 0. Any missing time points are set to the mean of their respective neighbours [ 10].
And notice they were sorted in context.
(In fact, they were sorted into groups randomly).
"I had to have my bed moved downstairs and a commode – I wasn't allowed home until they were sorted out.
Parcel post packages came along a conveyor belt and were thrown down a chute, according to state, where they were sorted by town.
They were sorted into two categories using US Census Bureau criteria: those from a high-poverty area and those from a low-poverty area.
"They have a real connection when they see the stones, not like if they were sorted out of a bag," Mr. Bale said.
They were sorted into groups of half a dozen or so each and lashed to metal frames that could be rolled up and down the block, like coat racks.
Neutrophils were labelled with CD45 (clone 30-F11), Ly6G (clone 1A8) and CD11b (clone M1/70) (Abcam, Cambridge, UK), and they were sorted by flow cytometry for subsequent experiments.
The mononuclear cell layer was isolated from the maternal blood and enriched for fetal nucleated red blood cells by flow cytometry with monoclonal antibodies to CD36 or CD71 and to glycophorin A. Eight samples were treated with CD45 monoclonal antibody coated magnetic beads before they were sorted to deplete the maternal sample of leukocytes (CD45+ cells).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com