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Three probiotic bacteria (L. paracasei, B. longum, B. breve) were successfully protected with γ-PGA when they were freeze dried.
They were freeze killed and stored at -28°C.
Once received at the center, fecal samples were weighed (wet weight) and placed in a freezer until they were freeze dried.
Shoot samples from each plant were placed in individual tubes and placed at -80°C for at least four hours, after which they were freeze dried under a vacuum at -75°C for two days.
If they were freeze dried, then the yeast was reconstituted with water and streaked onto supplemented yeast extract agar plates; (YES; 5% yeast extract, 3% glucose, 225 mg/L histidine, 225 mg/L adenine, 225 mg/L leucine, and 225 mg/L uracil), 2% agar Bacto agar (Becton Dickinson).
Similar(55)
The most unusual thing about the fossils was that they were freeze-dried; when rehydrated they resembled living specimens.
I believe they were frozen.
Once they were frozen, they could not be removed.
("But they were frozen, not like ours," Mr. Lao said. "They were terrible").
They clapped at the appropriate times as if they were freezing on the Mall in Washington.
At first they sang but soon the cold became so bad that they were freezing.
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