When C2C12 cultures reached 70 90 % confluency they were differentiated by changing the media to differentiation medium consisting of DMEM supplemented with 2 % horse serum, 200 U/ml penicillin and 200 µg/ml streptomycin.
Because many people received the same name (given name), they were differentiated by surnames (for example, John Redhead, John Hunter, John Scott).
"I couldn't quite grasp why there were three fairs, how they were differentiated from each other," said Jessica Silverman, of the Silverman Gallery in San Francisco, who opted out of the action.
The MSCs were cultured in media containing various concentrations (0 500 μM) of l-ascorbate-2-phosphate (Asc-2-P) for 2 weeks, following which they were differentiated into adipocytes and osteoblasts.
Calu-3 is a transformed continuous cell line originally isolated from a human pulmonary adenocarcinoma and they were differentiated in transwell culture inserts for one week only.
TG25 cells carry a GFP knock-in at the Olig2 genes; GFP in the cells is turned on when they were differentiated [49], thus serving as a positive control.
