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To determine if the MBP/exo and MBP/endo had the complete and active chitinase protein, they were cleaved using the Factor Xa protease (New England Biolabs).
But the question raised was whether the compounds were processed by the enzyme and then released from the solid-phase or whether they were cleaved and then processed by the enzyme.
They were cleaved from the resins and defracted by treatment with trifluoroacetic acid containing ethanedithiol, water triisopropilsilane and anisole (93 : 2.5 : 2 : 1.5 : 1).
The results demonstrated that the TEV protease worked effectively on both constructs under a wide range of conditions, since they were cleaved completely in different buffers, either coupled to beads or in solution.
The series of RPA32172 270 spectra acquired prior to and after incubation with Proteinase K shows that 30 residues disappeared from the spectrum as they were cleaved by the protease.
Although the original anti-ROS-CII specificity of 1-11E was maintained in 1-11E/vIL-10, the in vitro vIL-10 bioactivity of 1-11E/vIL-10 and C7/vIL-10 fusion proteins was very low until they were cleaved by MMP-1, indicating that vIL-10 was latent before the MMP-1 digestion.
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The ProCas9s can only be activated when they're cleaved by matching proteases, meaning they can sense and respond to a specific type of cell, said the researchers.
Those prohormones are stored in granules, within which they are cleaved into active hormones and are secreted into the circulation.
Others, which provide clearer images, don't emit any light when they're injected in the body but light up when they are cleaved by specific proteins in the body.
One approach to making Env trimers is to ensure that they are cleaved at the gp120 gp41 border, but stabilized by other, engineered substitutions such as intra-subunit disulfide bonds (SOS and SOSIP gp140 proteins).
They are cleaved at the extracellular domain to release the soluble form of ACE into the extracellular fluid, including the blood [29 31].
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